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. 2010 Jun;9(6):952–959. doi: 10.1128/EC.00005-10

Fig. 7.

Fig. 7.

Association of recombinant GST-Pfcrk-3 with histone H1 kinase activity. (A) Pulldown experiments using wild-type GST-Pfcrk-3. One microgram of GST-Pfcrk-3 (lanes 2 and 4) or GST alone (lanes 1 and 3) bound to glutathione-agarose beads was incubated in parasite extract (lanes 1 and 2) or in parasite lysis buffer as a negative control (lanes 3 and 4). The beads were washed and resuspended in kinase assay buffer, and in vitro kinase assays were performed by addition of a reaction mixture containing radiolabeled ATP and histone H1. (B) Pulldown experiments using wild-type and kinase-dead GST-Pfcrk-3, followed by kinase assays. Portions (0.5 μg) of GST-Pfcrk-3 (lanes 1 and 2), GST-Pfcrk-3-K445M (lane 3), or GST alone (lane 4) bound to glutathione-agarose beads were incubated in parasite extracts (lanes 2 to 4) or in parasite lysis buffer as a negative control (lane 1). The beads were washed, and in vitro kinase assays were performed by addition of a reaction mixture containing the radiolabeled ATP and histone H1.