Fig. 1.

Effect of ACT1-regulated overexpression of CDR1, CDR2, or MDR1. (A) Intracellular [³H]fluconazole levels after incubation in 0.05 μM [³H]fluconazole for the times shown were highest in C. albicans DSY1050F cells transformed with pACT1 alone (empty vector), lower in C. albicans DSY1050F cells transformed with pACT1-CDR1 (CDR1), pACT1-CDR2 (CDR2), or pACT1-MDR1 (MDR1), and lowest in wild-type C. albicans SC5314. Data are means ± SD for 3 experiments. (B) Immunoreactive proteins of the sizes expected for Flag-tagged Cdr1p, Cdr2p, and Mdr1p, respectively, were demonstrated in Western blots of lysates of the pACT1-CDR1, pACT1-CDR2, and pACT-MDR1 transformants probed with anti-Flag antibodies but not in Western blots of lysates of pACT1-transformed controls (empty vector) (100 μg total protein per lane).