Fig. 6.

Secondary peaks of calflagin RNAi parasitemia are associated with alternative VSG expression. Mice were inoculated with wild-type or calflagin RNAi blood form parasites expressing the MITat 1.2 VSG (inoculum, left). Parasitemias were followed, and the VSG expressed at the first or second relapse (arrows) was determined by cDNA cloning and sequencing. MITat 1.2 immunofluorescence was negative in all cases (right). The specific VSG expressed in each of these animals is indicated in the parasitemia plot.