Fig. 8.

Surface antibody clearance is unaffected by calflagin depletion. Live bloodstream form parasites were labeled at 4°C with anti-VSG antibody and then incubated at 37°C for various lengths of time (0 to 600 s) to allow surface clearance of antibody prior to secondary labeling and fixation. Flow cytometric analysis was performed, and the resulting MFIs of the wild-type and calflagin-depleted cells in the positive population were compared. Neither the initial staining nor the rate of clearance differed significantly between groups. More than 10,000 live nucleated cells were analyzed (n = 3).