GalXM immunofluorescence staining was dependent on growth phase in C. neoformans. Strains B3501 (serotype D) (A), 24067 (serotype D) (B), and H99 (serotype A) (C) each showed comparable patterns in both growth kinetics and GalXM labeling as a function of culture time. Maximum GalXM staining occurred in the stationary phase. Panels D, E, and F show the frequency of dots per cell and as a function of time for strains B3501, 24067, and H99, respectively. Fig. 7 shows representative examples of the cells counted in this assay.