Fig. 4.

Genome rearrangements mediated by translocations at MRS sequences in C. albicans isolates. (A to C) CHEF karyotype diagrams of strains 1006, WO-1, and WO-2 (118). WO-1 underwent three reciprocal translocation events, resulting in 6 new fusion chromosomes. All translocations occurred at or near the MRS, resulting in identical SfiI digestion patterns between 1006 and WO-1 (35). WO-2 (derived from WO-1) underwent multiple chromosome nondisjunction events, resulting in loss of the full-length Chr7 homolog and loss of the fusion Chr5,6 (114). (D) aCGH analysis indicates that strain WO-1 does not contain obvious whole-chromosome or segmental aneuploidies, although some isolates of WO-1 do carry a trisomy of Chr1 (149). (E) In contrast, WO-2 carries a total of four segmental aneuploidies, including segmental trisomy of Chr4 (the result of duplication of the Chr7,4 fusion chromosome) and segmental monosomy of Chr5, -6, and -7 (after loss of the full-length Chr7 homolog and loss of the Chr5,6 fusion chromosome). Note that the transition points for the segmental aneuploidies all occur at or very close to the MRS (pink ovals). aCGH analyses of WO-1 and WO-2 have not been published previously.