FIG. 8.
Siderophore production and growth phenotype of xsuA mutants of X. oryzae pv. oryzae constructed by integration of suicide plasmid pK18mob. (A) Strains depicted are BXO43 (wild-type strain), BXO2235 (xsuA2::pK18mob; a nonpolar mutation), and BXO2236 (xsuA3::pK18mob; a polar mutation). X. oryzae pv. oryzae strains were grown either on PSA-CAS medium or on PSA-CAS medium supplemented with 100 μM DP. Siderophore production is evident as a halo around the colonies. (B) BXO2235 and BXO2236 are deficient for growth under iron-limiting conditions. Cell numbers were estimated in X. oryzae pv. oryzae cultures grown in PS medium and PS-100 μM DP medium at 0 h and after 48 h of growth.