TABLE 2.
Primers used in this study
| Primer use and name | Sequence (5′-3′) | Reference |
|---|---|---|
| Construction of algU mutant and complementing plasmid | ||
| AlgU1 | ATATGAATTCTGGAGCCCTAGTATATAGAAGGG | This study |
| AlgU2 | ATTACTGCAGGTTAGCATGAAAGCTCCTCT | This study |
| AlgU3 | AAGCAATCGACAAAGCTCTG | This study |
| AlgU4 | ATATAAGCTTAAGCAAAAGCAACAGGGA | This study |
| AUCP1 | ATTAGGATCCTGTCATCGGCAGGCGTCATCA | This study |
| AUCP2 | ATTAGAATTCTGGCATTTGCCGCTGTGTCAG | This study |
| Quantification of mRNA levels by qRT-PCR | ||
| 16SF | CAGGATTAGATACCCTGGTAGTCCAC | 10 |
| 16SR | GACTTAACCCAACATCTCACGACAC | 10 |
| PelA1 | TGTCGGGTATCTGAAAGAGCAG | This study |
| PelA2 | GACCGACAGATAGGCGAAGG | This study |
| PslA1 | GTTCTGCCTGCTGTTGTTCATG | This study |
| PslA2 | AGGTAGGGAAACAGGCCCAG | This study |
| LecA1 | TGGAAAGGTGAGGTTCTGGC | This study |
| LecA2 | AATCGACGTTACCTGCCCTG | This study |
| LecB3 | ACCAATAACGCCGTCATCG | This study |
| LecB4 | CTGTACCTTGCCACTGCTGC | This study |
| Fhp1 | AATTACCTGCATGACCGGGTC | This study |
| Fhp2 | CGGAAACAGATCCAGCTCGT | This study |
| PpyR1 | TTTCCTCGACCGCTACCTGA | This study |
| PpyR2 | AGCGCATGGGAAAACACC | This study |
| Promoter mapping by 5′-RACE PCR | ||
| gsp1lecB | ATTTGCTGTGCCGAGACCAGA | This study |
| gsp2lecB | TTATTGGTGCTTTGCCCGC | This study |
| neslecB | AGGCGGTGACGCCGAACCGG | This study |