FIG. 2.

Overexpression of type 1 fimbriae inhibits biofilm formation on cholesterol-coated surfaces, while normal or decreased expression of type 1 fimbriae has no effect. Biofilm formation by serovar Typhimurium strains grown with 3% crude ox bile extract on cholesterol-coated Eppendorf tubes was examined. Crystal violet-stained TBA biofilms were extracted with acetic acid, and absorbance at 570 nm was measured. *, statistically significantly different (P < 0.005) based on a two-tailed Student t test. OD570, optical density at 570 nm.