FIG. 4.

Expression of purified flagellum proteins from serovar Typhimurium wild-type, fliC mutant, fljB mutant, and fliC fljB mutant strains adhering to cholesterol-coated wells. An ELISA was performed using antiflagellum or anti-FliC monoclonal antibodies, and binding to cholesterol was quantified by measuring the bound secondary HRP-conjugated substrate using the optical density at 415 nm (OD415). Asterisks indicate statistically significant differences based on a two-tailed Student t test (*, P < 0.05; **, P < 0.005). Ab, antibody.