TABLE 3.
Binding quantification assaya
| Strain | Growth conditions | Live | Dead |
|---|---|---|---|
| Serovar Typhimurium | LB medium | + | + |
| wild type | LB medium + 3% bile | + | + |
| Serovar Typhimurium | LB medium | − | − |
| flhC mutant | LB medium + 3% bile | − | − |
a
A 3-h binding quantification assay was performed with cholesterol-coated wells containing live or dead serovar Typhimurium wild-type and flhC mutant strains. Crystal violet-stained material was extracted with acetic acid, and the optical density at 570 nm was determined for quantification. +, binding equivalent to wild-type binding under appropriate conditions; −, severe defect in or complete loss of adherence (values that were 0 to 10% of the wild-type values).