FIG. 10.
Activation of biofilm accumulation by Mlc is partially dependent on EIIAGlc: quantification of biofilm accumulation and total growth by wild-type V. cholerae (WT) and ΔMlc, ΔEIIBCGlc ΔMlc, and ΔEIIAGlc ΔEIIBCGlc ΔMlc mutants rescued with either a control vector (pCTL) or a plasmid carrying a wild-type mlc allele (pMlc). Biofilm formation by ΔMlc (P < 0.0001), ΔEIIBCGlc ΔMlc (P < 0.0001), and ΔEIIAGlc ΔEIIBCGlc ΔMlc (P = 0.002) mutants rescued with the plasmid carrying the wild-type mlc allele was significantly greater than that by mutants carrying a control vector. The error bars indicate standard deviations.