EIIA homologs EIIAGlc, EIIANtr1, and EIIANtr2 regulate biofilm formation: quantification of total growth and biofilm accumulation by wild-type V. cholerae, as well as strains having mutations in each of the 10 genes encoding EIIA domains. The biofilm accumulation by the ΔEIIAGlc (P < 0.0001), ΔEIIANtr (P < 0.0001), and ΔVC1824 (P = 0.0004) mutants was significantly different from that by wild-type V. cholerae (WT). The error bars indicate standard deviations.