FIG. 8.
Evidence for involvement of Mlc and EIIBCGlc in pathway 2: quantification of total growth and biofilm accumulation by wild-type V. cholerae and various pathway 2 mutants. Biofilm accumulation by the ΔEI (P = 0.0008) or ΔEIIBCGlc (P = 0.0001) mutant was significantly greater than that by wild-type V. cholerae (WT). Biofilm accumulation by the ΔEIIAGlc or ΔMlc mutants was significantly less than that by wild-type V. cholerae (P < 0.0001). Biofilm accumulation by the ΔEIIAGlc ΔEIIBCGlc mutant (P = 0.04) or the ΔEIIAGlc ΔEIIBCGlc ΔMlc mutant (P = 0.001) was significantly greater than that by the ΔEIIAGlc mutant. Biofilm accumulation by the ΔEIIBCGlc ΔMlc mutant (P = 0.0006) or the ΔEIIAGlc ΔEIIBCGlc ΔMlc mutant (P = 0.0005) was significantly greater than that by the ΔMlc mutant. The error bars indicate standard deviations.