TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or phenotype | Reference |
|---|---|---|
| E. coli SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mul pir R6K; Kmr | 38 |
| V. cholerae strains | ||
| PW357 | MO10 lacZ::vpsLp→lacZ; Smr | 15 |
| PW751 | MO10 lacZ::vpsLp→lacZ ΔPTS; Smr | 17 |
| PW961 | MO10 lacZ::vpsLp→lacZ ΔEI; Smr | 17 |
| PW964 | MO10 lacZ::vpsLp→lacZ ΔHPr; Smr | 17 |
| PW954 | MO10 lacZ::vpsLp→lacZ ΔFPr; Smr | 16 |
| PW965 | MO10 lacZ::vpsLp→lacZ ΔHPr ΔFPr; Smr | 16 |
| PW836 | MO10 lacZ::vpsLp→lacZ ΔEIIAGlc; Smr | 17 |
| PW989 | MO10 lacZ::vpsLp ΔEIIBCGlc; Smr | 17 |
| PW988 | MO10 lacZ::vpsLp ΔEIIAGlc ΔEIIBCGlc; Smr | This study |
| PW996 | MO10 lacZ::vpsLp ΔEIIBCGlc ΔMlc; Smr | This study |
| PW994 | MO10 lacZ::vpsLp ΔEIIAGlc ΔEIIBCGlc ΔMlc; Smr | This study |
| PW993 | MO10 lacZ::vpsLp ΔMlc | This study |
| PW992 | MO10 lacZ::vpsLp→lacZ ΔEIIANtr1; Smr | 16 |
| PW991 | MO10 lacZ::vpsLp→lacZ ΔEIIANtr2; Smr | 16 |
| PW1002 | MO10 lacZ::vpsLp→lacZ VC1283::pGP704; Smr Apr | 16 |
| PW1003 | MO10 lacZ::vpsLp→lacZ VC1820::pGP704; Smr Apr | 16 |
| PW1004 | MO10 lacZ::vpsLp→lacZ VC1822::pGP704; Smr Apr | 16 |
| PW1005 | MO10 lacZ::vpsLp→lacZ VC1826::pGP704; Smr Apr | 16 |
| PW1006 | MO10 lacZ::vpsLp→lacZ VCA0245::pGP704; Smr Apr | 16 |
| PW1007 | MO10 lacZ::vpsLp→lacZ VCA1045::pGP704; Smr Apr | 16 |
| Plasmids used in rescue experiments | ||
| pBAD-TOPO-EI | pBAD-TOPO carrying the coding sequence of VC0965 | 17 |
| pBAD-TOPO-EI(H189A) | pBAD-TOPO carrying the coding sequence of VC0965 with an H-to-A mutation at position 189 | 17 |
| pBAD-TOPO-EIIAGlc | pBAD-TOPO carrying the coding sequence of VC0964 | 17 |
| pBAD-TOPO-EIIAGlc(H91A) | pBAD-TOPO carrying the coding sequence of VC0964 with an H-to-A mutation at position 91 | This study |
| pBAD-TOPO-EIIAGlc(H91D) | pBAD-TOPO carrying the coding sequence of VC0964 with an H-to-D mutation at position 91 | This study |
| pBAD-TOPO-mlc | pBAD-TOPO carrying the coding sequence of VC2007 | This study |
| pBAD-TOPO-EIIANtr1 | pBAD-TOPO carrying the coding sequence of VC2531 | This study |
| pBAD-TOPO-EIIANtr1(H66A) | pBAD-TOPO carrying the coding sequence of VC2531 with an H-to-A mutation at position 66 | This study |
| pBAD-TOPO-EIIANtr1(H66D) | pBAD-TOPO carrying the coding sequence of VC2531 with an H-to-D mutation at position 66 | This study |
| pBAD-TOPO-EIIANtr2 | pBAD-TOPO carrying the coding sequence of VC1824 | This study |
| pBAD-TOPO-EIIANtr2(H172A) | pBAD-TOPO carrying the coding sequence of VC1824 with an H-to-A mutation at position 172 | This study |
| pBAD-TOPO-EIIANtr2(H172D) | pBAD-TOPO carrying the coding sequence of VC1824 with an H-to-D mutation at position 172 | This study |