FIG. 6.
Time-lapse microscopy of GFP-SpoIIP. Sporulating cells were incubated on agarose pads at 30°C and membranes were stained with FM 4-64. Image sequences were initiated at t2.5 after the initiation of sporulation by resuspension and the first image was set to t = 0 min. Minutes after t = 0 is indicated in lower right of GFP image. The upper panels show FM 4-64 membranes merged with GFP-SpoIIP; the lower panels show GFP-SpoIIP alone. (A) GFP-SpoIIP in wild type (KP1129). GFP-SpoIIP is localized evenly to the leading edges throughout migration and becomes diffuse after the completion of membrane migration. (Bi and ii) Two examples of GFP-SpoIIP in a SpoIIDD210A background (KP1132). Localization corresponds with advancement of the membrane, and a faster membrane arm is indicated by arrowheads (i). GFP-SpoIIP is localized unevenly to the leading edges or diffuse throughout blebs; diffuse GFP-SpoIIP is indicated by arrows (ii).