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. 2010 Apr 2;192(12):3093–3102. doi: 10.1128/JB.00133-10

FIG. 3.

FIG. 3.

Disruption of the chromosomal flgA1-flgA2 and pibD loci in H. volcanii strains H98 and H53. PCR amplification was performed using primers against the flanking regions. (A) Amplification of approximately 700 nucleotides upstream and 700 bp downstream of flgA1-flgA2, using template DNA isolated from the wild-type (H98 or H53) or the ΔflgA1 ΔflgA2 H. volcanii strains (MT1 or MT2, respectively). (B) Amplification of approximately 700 nucleotides upstream and 700 bp downstream of pibD, using template DNA isolated from the wild-type (H98 and H53) or the ΔpibD H. volcanii strains (MT3 o MT4, respectively). As expected, the amplicons are approximately 1,300 and 1,100 nucleotides smaller for the ΔflgA1 ΔflgA2 and ΔpibD deletion strains, respectively, than the amplicons obtained using DNA isolated from the parental strains.