TABLE 1.
Strains and plasmids
| Plasmid or strain | Relevant properties or description | Reference or source |
|---|---|---|
| Plasmids | ||
| pTA131 | Ampr; pBluescript II with BamHI-XbaI fragment from pGB70 containing pfdx-pyrE2 | 6 |
| pRV1-ptna | Ampr Novr ptna | 31 |
| pMT1 | pTA131 containing chromosomal flgA1-flgA2 region | This study |
| pMT2 | pTA131 containing chromosomal pibD region | This study |
| pMT3 | pRV1-ptna containing flgA1-flgA2-His | This study |
| pMT4 | pRV1-ptna containing pibD-His | This study |
| pMT5 | pRV1-ptna containing Hvo_A0632-His; type IV pilin-like gene | This study |
| pMT6 | pRV1-ptna containing Hvo_2451-His; type IV pilin-like gene | This study |
| Strains | ||
| E. coli | ||
| DH5α | F− 80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Invitrogen |
| DL739 | MC4100 recA dam-13::Tn9 | 13 |
| H. volcanii | ||
| H98 | ΔpyrE2 ΔhdrB | 6 |
| H53 | ΔpyrE2 Δtrp | 6 |
| MT1 | H98 ΔflgA1 ΔflgA2 | This study |
| MT2 | H53 ΔflgA1 ΔflgA2 | This study |
| MT3 | H98 ΔpibD | This study |
| MT4 | H53 ΔpibD | This study |
| MT5 | H98 containing pMT3 | This study |
| MT6 | H98 containing pMT4 | This study |
| MT7 | MT3 containing pMT3 | This study |
| MT8 | MT3 containing pMT4 | This study |
| MT9 | H98 containing pMT5 | This study |
| MT10 | H98 containing pMT6 | This study |
| MT11 | MT3 containing pMT5 | This study |
| MT12 | MT3 containing pMT6 | This study |
| aglB strain | H53 ΔaglB | 1 |