TABLE 2.

Primers used for PCR amplification

Primer name	Sequences (5′-3′)a	Target sequence
FlgA1/2KO up for HindIII	GCCGTAAAAGCTTGCTCGTCCTCGACTC	705 bp upstream of flgA1 start codon, extension toward gene
FlgA1/2KO up rev EcoRI	TTACTTGGAATTCGTAACCCGGCACCGA	1 bp upstream of flgA1 start codon, extension away from gene
FlgA1/2KOd for BamHI	ACGTCGGGATCCAGATTTCGTGGGTTT	1 bp downstream of flgA2 stop codon, extension away from gene
FlgA1/2KOd rev XbaI	GCGACGTCTAGAACGTTCGTCCTCGTC	705 bp downstream of flgA2 stop codon extension toward gene
PibDKOup for HindIII	TTATTAAGCTTCTCTCGCGCCGGGATC	695 bp upstream of pibD start codon, extension toward gene
PibDKOup rev EcoRI	GCAGTGAATTCTACCCGGAGACTGGGG	1 bp upstream of pibD start codon, extension away from gene
PibDKOd for BamHI	ATTATGGATCCGTCGCTGCGGAGTCG	1 bp downstream of pibD stop codon, extension away from gene
PibDKOd for XbaI	GGATAGTCTAGAGTCGTCCGGCGGGTC	705 bp downstream of pibD stop codon extension toward gene
FlgA1/2for	CGCCCGTGCGAGAGGTGATT	71 bp downstream of flgA1 start codon, extension toward gene
FlgA1/2rev	CGAGGCGGACCGTCAGGAAA	262 bp upstream of flgA2 stop codon, extension toward gene
PibDfor	TTCGCCTTCGGACTGCTCGGT	283 upstream of pibD start codon, extension toward gene
PibDrev	CGCGACGAACTCTGGGAGAAG	255 downstream of pibD stop codon, extension toward gene
FlgA1for NdeI	ATTAATCATATGTTCGAAAACATCAACGAA	1 bp downstream of flgA1 start codon, extension toward gene
FlgA2 6-His EcoRI rev	TATATTGAATTCTCAGTGATGGTGATGGTGAT     GCGGGCCGCCCAGTTCGACGGGTT	1 bp upstream of flgA2 stop codon, extension toward gene
PibDfor NdeI	GCTATCCATATGTTCGGTATCGGCACG	1 bp downstream of pibD start codon, extension toward gene
PibD 6-His EcoRI rev	TATATTGAATTCTCAGTGATGGTGATGGTGAT     GCGGGCCGCCGACGATACCGAGCGCG	1 bp upstream of pibD stop codon, extension toward gene
Hvo_A0632 forNdeI	CCCGCGCATATGGATATCAAGAAATTCCTTAG	1 bp downstream of Hvo_A0632 start codon, extension toward gene
Hvo_A0632 6-His EcoRI rev	TATATTGAATTCTCAGTGATGGTGATGGTGAT     GCGGGCCGCCGTGCGTGTACGTTTG	1 bp upstream of Hvo_A0632 stop
Hvo_2451 for NdeI	AATTACCATATGCAACTCAAACATCTCTTCAC	1 bp downstream of Hvo_2451 start codon, extension toward gene
Hvo_2451 6-His EcoRI rev	GTAATTGAATTCTCAGTGATGGTGATGGTGA     TGCGGGCCGCCTCCGTTGTAGGTCCA	1 bp upstream of Hvo_2451 stop

^aRestriction endonuclease sites are underlined.