TABLE 3.
Phenotypic characterization of H. volcanii ΔflgA1 ΔflgA2 and ΔpibD deletion strains
| Straina | Value for the characteristic on the indicated medium |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Minimal doubling time (h)b,c |
Competition assay (H53/H98) |
Conjugation frequency |
Ca2+-dependent aggregationb |
Motility (halo diam [cm] after 72 h)b,d |
Adhesionb |
|||||||
| CX | CA | CX | CA | CA + filter | Liquid CA | CX | CA | CX | CA | CX | CA | |
| WT | 3.5 | 4.5 | 50/50 | 50/50 | 10−4 | 10−8 | − | + | 0.9 | 0.7 | + | + |
| ΔflgA1 ΔflgA2 strain | 3.5 | 4.5 | 50/50 | 50/50 | 10−4 | 10−8 | − | + | − | − | + | + |
| ΔpibD strain | 3.5 | 4.5 | 50/50 | 50/50 | 10−4 | 10−8 | − | + | − | − | − | − |
a
Both the H53 and H98 backgrounds were used for all strains.
b
No differences were observed whether H98 or H53 background was used.
c
Standard deviation, 0.5 h.
d
Standard deviation, 0.22 and 0.29 cm in CX and CA motility media, respectively.