FIG. 1.

Maximum-likelihood (ML) trees showing the phylogenetic relationships of Mn(II)-oxidizing fungi (a) or bacteria (b) isolated from Mn treatment systems using 18S rRNA and 16S rRNA gene analysis, respectively. Isolates from this study are shown in bold and boxed, and previously identified Mn(II)-oxidizing isolates are in bold and unboxed. Both ML trees were constructed with the PhyML software package (19) in ARB using the generalized time reversible (GTR) nucleotide substitution model with 1,000 bootstrap replicates. Bootstrap values for nodes with greater than 50% support are displayed as percentages. GenBank accession numbers are in parentheses. Scale bar, 0.05 (a) or 0.10 (b) substitutions per nucleotide site.