TABLE 2.
Nearest relatives in GenBank to the Bacteroidales 16S rRNA gene sequences obtained from groundwater and unchlorinated tap water from plant C using different primer setsa
| Primer set used, source of sample, and OTUsb | GenBank sequence accession no. | Source of sequence (GenBank sequence accession no.) | Similarityc | Nearest cultivated bacterium in GenBank (sequence accession no.) | Similarity |
|---|---|---|---|---|---|
| AllBac | |||||
| Extraction well 1 (3/6) | GQ169588 | Rhizosphere (EF605968) | 108/108 | Prevotella oralis (AY323522) | 105/108 |
| Extraction well 1 (3/6) | GQ169589 | Water from watershed (DQ886209) | 108/108 | Tannerella forsythia(AB035460) | 107/108 |
| Extraction well 2 (1/6) | GQ169590 | Phyllosphere Brazilian forest (DQ221468) | 108/108 | Tannerella forsythia(AB035460) | 106/108 |
| Extraction well 2 (5/6) | GQ169591 | Bovine rumen (EU348207) | 108/108 | Tannerella forsythia(AB035460) | 106/108 |
| Extraction well 3 (1/6) | GQ169592 | Phyllosphere Brazilian forest (DQ221468) | 108/108 | Prevotella oralis (AY323522) | 104/108 |
| Extraction well 3 (5/6) | GQ169593 | Prevotella corporis (L16465) | 108/108 | Prevotella corporis (L16465) | 108/108 |
| Raw water (3/6) | GQ169594 | Spitsbergen permafrost (EF034756) | 108/108 | Tannerella forsythia(AB035460) | 106/108 |
| Raw water (3/6) | GQ169595 | Hindgut beetle larvae (FJ374179) | 108/108 | Tannerella forsythia(AB035460) | 107/108 |
| Tap water (6/6) | GQ169596 | Prevotella timonensis (DQ518919) | 108/108 | Prevotella timonensis (DQ518919) | 108/108 |
| Prevotella buccalis (L16476) | Prevotella buccalis (L16476) | ||||
| Prevotella ruminicola (AF218617) | Prevotella ruminicola (AF218617) | ||||
| Bacteroides vulgatus (NC_009614) | Bacteroides vulgatus (NC_009614) | ||||
| TotBac | |||||
| Extraction well 1 (1/10) | GQ169597 | Deep subsurface groundwater (AB237705) | 339/369 | Salinimicrobium terrae (EU135614) | 315/370 |
| Extraction well 1 (1/10) | GQ169598 | Songhuajiang River sediment (DQ444125) | 363/377 | Paludibacter propionicigenes (AB078842) | 357/376 |
| Extraction well 1 (4/10) | GQ169599 | Freshwater pond sediment (DQ676447) | 352/360 | Paludibacter propionicigenes (AB078842) | 313/372 |
| Extraction well 1 (4/10) | GQ169600 | Pine River sediment (DQ833352) | 364/371 | Bacteroides oleiciplenus (AB490803) | 334/375 |
| Extraction well 2 (4/10) | GQ169601 | Groundwater (AF273319) | 364/371 | Xanthobacillum maris (AB362815) | 338/375 |
| Extraction well 2 (6/10) | GQ169602 | Human saliva (AB028385) | 381/382 | Prevotella intermedia (AY689226) | 380/382 |
| Extraction well 3 (1/10) | GQ169603 | Pig manure (AY816766) | 354/377 | Bacteroides thetaiotaomicron (AE015928) | 311/380 |
| Extraction well 3 (3/10) | GQ169604 | Pig manure (AY816867) | 371/376 | Butyricimonas virosa (AB443949) | 307/379 |
| Extraction well 3 (6/10) | GQ169605 | Swedish lake (AY509350) | 343/362 | Parabacteroides distasonis (AB238927) | 320/374 |
| Raw water (10/10) | GQ169606 | Prevotella timonensis (AF218617) | 378/379 | Prevotella timonensis (AF218617) | 378/379 |
| Tap water (1/10) | GQ169607 | Deep subsurface groundwater (AB237705) | 338/369 | Salinimicrobium terrae (EU135614) | 312/370 |
| Tap water (2/10) | GQ169608 | Yukon River, AK(FJ694652) | 367/372 | Psychroserpens burtonensis (U62913) | 312/375 |
| Tap water (7/10) | GQ169609 | Deep subsurface groundwater (AB237705) | 341/369 | Salinimicrobium terrae (EU135614) | 315/370 |
a
Primer sets AllBac (20) and TotBac (12) were used in PCRs of samples, and GenBank was searched for relatives using BLAST.
b
OTUs are indicated by the values in parentheses (number of sequences belonging to the OTU/total number of sequences analyzed).
c
Number of base pairs identical in both sequences/total number of base pairs in sequences.