TABLE 2.

Expression of STI gene-lacZ fusions after 7 days of incubation in batch culture

Strain	Functional group	Gene interrupted by lacZ fusion and/or its product or function	LacZ activitya (SD)
AGCV19	Envelope	ostA	2,389 (890)
AGCV23	Gene expression	trxAb	1,534 (222)
AGCV12	DNA metabolism	pilU	1,025 (388)
AGCV1	Metabolism	argS	1,014 (63)
AGCV28	Anabolism	gshA	808 (218)
AGCV7	Anabolism	leuA	718 (87)
AGCV18	Anabolismc	kdsB	682 (54)
AGCV25	Unknown	ACIAD3229, CHP	652 (126)
AGCV13	Metabolism	purA	608 (271)
AGCV10	Unknown	ACIAD0865, CHP	556 (346)
AGCV26	Unknown	ACIAD3343, CHP	427 (54)
AGCV8	Unknown	ACIAD0615, CHP	417 (15)
AGCV21	Unknown	ACIAD2746 but reversed,d CHP	390 (93)
AGCV2	Unknown	ACIAD0167, CHP	367 (48)
AGCV4	Gene expression	infB	359 (54)
AGCV14	DNA metabolism	Putative methylase	282 (131)
AGCV11	Gene expression	fusA	255 (132)
AGCV15	Catabolism	mdcA	233 (131)
AGCV5	Envelope	phaAB	225 (29)
AGCV20	Envelope	tolB	224 (15)
AGCV29	Envelope	Putative outer membrane protein	224 (15)
AGCV22	Gene expression	Putative RNA-binding protein	207 (62)
AGCV27	Anabolism	aroK	173 (25)
AGCV9	DNA metabolism	Putative mutT	157 (25)
AGCV3	Catabolism	atpA	133 (34)
AGCV17	Unknownb	ACIAD1960, CHP	92 (21)
AGCV24	Catabolism	almA	56 (3)
AGCV16	Catabolism	Putative enoyl-coenzyme A hydratase	50 (14)
AGCV6	DNA metabolism	recD	39 (14)
AGCV30	Gene expression	23S rRNA	11 (3)

^anmol o-nitrophenol/30 min/10⁶ CFU; see the supplemental material.

^blacZ fusion is in the same orientation as trxA but is inserted after the stop codon, before the next gene (rho).

^cSurrounding genes that could be part of an operon with kdsB or ACIAD1960 suggest a role in transport.

^dlacZ gene is inserted opposite the direction of the open reading frame.