TABLE 2.
Phylogeny of Stege Marsh dsrAB sequences
| Clade | Cluster | OTUa | Most related dsrAB sequence(s) (GenBank accession no.) | % Similarity |
|---|---|---|---|---|
| 1 | Desulfobacteraceae | D18 | PIMO2B05 (AY741564), Desulfosarcina variabilis (AF191907) | 71 |
| F24 | PIMO3C12 (AY741572), Desulfosarcina variabilis (AF191907) | 78 | ||
| D41 | Desulfococcus multivorans (U58126) | 83 | ||
| A26 | PIMO2D08 (AY741561) | 81 | ||
| A19 | VN3 (AY953395) | 70 | ||
| O12 | Desulfosalina propionicus PropA (DQ386237) | 85 | ||
| A36 | VN3 (AY953395) | 67 | ||
| F8 | VN3 (AY953395) | 55 | ||
| 2 | Desulfovibrionaceae | O1 | Desulfovibrio fructosivorans (AB061538) | 89 |
| O21 | Desulfovibrio fructosivorans (AB061538) | 83 | ||
| C6 | Desulfovibrio fructosivorans (AB061538) | 87 | ||
| 3 | Desulfobacterium | A18 | Desulfobacterium anilini (AF482455) | 81 |
| 4 | Desulfobulbaceae | D37 | PIMO8H06 (AY741580) | 99 |
| F21 | PIMO8H06 (AY741580) | 81 | ||
| O13 | PIMO3D11 (AY741574) | 81 | ||
| 5 | Syntrophobacteraceae/Desulfoarculus | A38 | Sbll-40 (AY167465) | 82 |
| D8 | Desulfoarculus baarsii (AF334600) | 70 | ||
| F25 | Guaymas B01P021 (AY197431) | 90 | ||
| 6 | Deeper branches | O15 | VN10 (AY953402) | 62 |
| D4 | VN10 (AY953402) | 68 | ||
| A21 | Sbll-36 (AY167469) | 45 | ||
| X4 | Desulfosporosinus orientis (AF271767) | 71 |
a
A, AMD pond; C, pyrite cinder enrichment culture (DSM410 amended with ∼1 mM fumarate); D, upper tidal slough; F, lower tidal slough; O, acid sulfate soils (dredged AMD pond sediments); U, 132 μM Cu(II) enrichment culture (modified MTM [88]); X, ∼1.2 M NaCl in DSM410 medium amended with ∼10 mM lactate.