FIG. 1.

Effect of NaCl concentration on the ability of wild-type V. parahaemolyticus RIMD2210633 to grow under mild, sublethal acid stress (pH 5). Vibrio parahaemolyticus was grown aerobically at 37°C in either LB with 3% NaCl (closed triangles), LB with 1% NaCl (open circles), or LB with 0.5% NaCl (closed circles) at pH 7 (A) or pH 5 (B). The organism was also grown in LB with 3% KCl at pH 5 (C), and growth was determined at OD₅₉₅. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations. An unpaired Student t test was used to infer statistical difference between cells grown in 3% NaCl and cells grown in either 1% or 0.5% NaCl for 24 h. *, P < 0.02; **, P < 0.002; ***, P < 0.0001.