FIG. 3.

Effect of high- and low-temperature stress on the growth and survival of V. parahaemolyticus RIMD2210633 at different NaCl concentrations. The organism was grown in either LB with 1% NaCl at pH 7 or LB with 3% NaCl at pH 7 at 42°C for 24 h (A). To study the role NaCl plays in low-temperature survival, exponential cultures of V. parahaemolyticus grown in either LB with 1% NaCl at pH 7 or LB with 3% NaCl at pH 7 were subjected to −20°C for 24 h (B). Survivability was determined by dividing the surviving population after 24 h by the initial population. All cultures were grown in triplicate, and each experiment was performed at least twice. Error bars indicate standard deviations. An unpaired Student t test was used to determine statistical differences between cells grown in 3% NaCl and cells grown in 1% NaCl. *, P < 0.0001.