FIG. 7.

Cytotoxicity of V. parahaemolyticus toward Caco-2 cells. Wild-type cells and ΔtoxRS cells were grown in LB with 1% NaCl at pH 7 or LB with 3% NaCl at pH 7 before being incubated with Caco-2 cells at an MOI of 100:1. Each condition was examined in triplicate, and the experiment was repeated at least twice. Error bars indicate standard deviations. The paired t test was performed to determine statistical difference between the samples tested. The asterisk denotes significant difference between results for wild-type cells grown in LB with 1% NaCl or ΔtoxRS cells grown in either LB with 1% NaCl or LB with 3% NaCl (P < 0.01) compared with results for wild-type cells grown in LB with 3% NaCl.