TABLE 1.
Bacterial strains and plasmids used in this study
| Strain, genotype, or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Vibrio parahaemolyticus | ||
| RIMD2210633 | O3:K6 clinical isolate | 19 |
| ΔtoxRS | RIMD2210633 ΔtoxRS(VP0812-813) | This study |
| ΔrpoS | RIMD2210633 ΔrpoS (VP2553) | This study |
| Escherichia coli | ||
| DH5α λpir | Δlac pir | |
| β2155 DAP | ΔdapA::erm pir for bacterial conjugation | |
| DH5α λpir ΔtoxRS | DH5α λpir containing pDS132ΔtoxRS | This study |
| DH5α λpir ΔrpoS | DH5α λpir containing pDS132ΔrpoS | This study |
| β2155 DAP-ΔtoxRS | β2155 harboring pDS132ΔtoxRS | This study |
| β2155 DAP-ΔrpoS | β2155 harboring pDS132ΔrpoS | This study |
| Plasmids | ||
| pDS132 | Suicide plasmid; Cmr; SacB | 31 |
| pDS132ΔtoxRS | pDS132 harboring truncated toxRS region | This study |
| pDS132ΔrpoS | pDS132 harboring truncated rpoS gene | This study |