TABLE 2.

Primers used in this study

Use and primer	Sequence (5′-3′)	Tm (°C)	Product size (bp)
Splice overlap extension PCR
SOEAtoxRS	ACC GTA GAA CCG TGA TTT AGG	54	289
SOEBtoxRS	ATG GCG ATT ACA TTC GCG T	55
SOECtoxRS	ACG CGA ATG TAA TCG CCA T	67	353
	TTG AGT CTG AAG AAA GCG GG
SOEDtoxRS	CGG CAC CAA ATT TCT ACT TG	52
SOEArpoS	TCA GCT CAG CTT AAT CCC TGA	56	507
SOEBrpoS	CCT GAC GCA AAC TCT GA	55
SOECrpoS	TCA GAG TTG TTT GAG TCA GG	68	315
	C GTC AAACTCTTCACGGACA
SOEDrpoS	ATT GCG ACC ATG GGT AGT TC	55
SOEFLtoxRSF	AGC CAC TTT ATG AGT GCC TA	53	1,840
SOEFLtoxRSR	GGA CGA CTT TGT GAT TTA GC	51
SOEFLrpoSF	ACA GCA TTG CTT TCA TGA AC	55	1,950
SOEFLrpoSR	AAA GAG CCG CCA AAG AAA GT	55
Real-time PCR
cadA Forward	TTG TAT GCC TCA GTC GCT TG	55	138
cadA Reverse	TCA CGC ATT TGC TAA CGA AC	54
toxR Forward	GAG ATT CCG CTG GGT TTG TA	55	101
toxR Reverse	TGT GGC TTC TGC TGT GAA TC	55.5
rpoS Forward	ATT TGA CGT ACA CGC TCA CG	55.5	165
rpoS Reverse	CGT CTT TGA TCC ATT GGT TG	52
16S Forward	ACG GCC TGG GGA GTA CGG TC	60	234
16S Reverse	TTG CGC TCG TTG CGG GAC TT	60