Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Real-time PCR primers and probes used in this study
| Gene region and primer or probe | Sequence (5′→3′)a |
|---|---|
| mcyB region | |
| Primers | |
| 30F | CCTACCGAGCGCTTGGG |
| 108R | GAAAATCCCCTAAAGATTCCTGAGT |
| Probe | FAM-CACCAAAGAAACACCCGAATCTGAGAGG-TAMRA |
| PC gene region | |
| Primers | |
| 188F | GCTACTTCGACCGCGCC |
| 254R | TCCTACGGTTTAATTGAGACTAGCC |
| Probe | Cy5-CCGCTGCTGTCGCCTAGTCCCTG-BHQ-2 |
FAM, 6-carboxyfluorescein; TAMRA, 6-carboxytetramethylrhodamine; Cy5, 5-indocarboxycyanine; BHQ-2, black hole quencher 2.
