Fig. 3.
The effect of tolfenamic acid on apoptotic cell death in KB cells. The KB cells were treated with dimethyl sulfoxide (DMSO) or various concentrations of 75 and 100 µM of tolfenamic acid for 48 h. A, The sub-G1 fraction was assessed by propidium iodide (PI) staining and flow cytometry analysis, Bar graph represents two-independent experiments; B, Annexin V-PI staining was carried out to detect apoptosis, Bar graph represents two-independent experiments; C, Fluorescence microscopy images of the 4'-6-diamidino-2-phenylindole (DAPI)-stained KB cells showing the concentration-dependent appearance of an apoptotic morphology in the tolfenamic acid-treated KB cells. White arrows point nuclear condensation and fragmentation.