Fig. 4.

(a) GLA induced cytotoxicity in Huh7 cells. Huh7 cells were untreated (Control) or treated with (GLA/Vit. E) or without (GLA) 100 µM Vitamin E in the presence of GLA 250 µM. Cell viability was measured by the WST-8 assay. ^#p<0.01. (b) GLA induced apoptosis cell death in Huh7 cells. Huh7 cells were untreated (Control) or treated with 250 µM GLA (GLA) for 24 h. The extent of apoptotic cell death was monitored by staining the nuclear DNA with a fluorescence DNA dye (Hoechst 33258) (n = 4). ^#p<0.01.