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A 3 mm thick primary melanoma lesion fixed in formalin and embedded in paraffin was sectioned and immunostained for Melan-A, Mitf, Wnt5A, Glut-1 and CD31. Examples of three separate regions including (i) nested, (ii) unstructured, and (iii) intermediate are shown in detail, as well as an overview of the complete section, for each marker.
