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VEGF promotes neurite extension from cortical neurons. In primary cultures of cortical neurons (>95% pure), neurofilament-positive neurites extend from neurons that are growing in clusters (A) or as single cells (C) in a serum-free environment. The addition of 50–100 ng/ml VEGF increases the diameter and length of neurites from clustered neurons significantly (B), with a stimulation of neurite outgrowth from the cell body by 30–40% (D).
