. 2010 Jun 21;8(6):1908–1919. doi: 10.3390/md8061908

Table 2.

PCR primers used for amplification of 16S rRNA gene for cyanobacteria identification and for the amplification of genes related to cyanotoxins production. A—Individual annealing temperature, B—Reference annealing temperature, bp = base pairs.

Primer	Sequence (5′→3′)	A	B	Size (bp)	Amplified gene	Reference
27F	AGAGTTTGATCCTGGCTCAG	52	60	780	16S rRNA	[22]
809R	GCTTCGGCACGGCTCGGGTCGATA	64	60	780	16S rRNA	[23]

740F	GGCYRWAWCTGACACTSAGGGA	-	50	754	16S rRNA	[22]
1494R	TACGGCTACCTTGTTACGAC	56	50	754	16S rRNA	[22]

mcyA-Cd F	AAAATTAAAAGCCGTATCAAA	51	59	297	Microcystin synthetase	[28]
mcyA-Cd R	AAAAGTGTTTTATTAGCGGCTCAT	43	59	297	Microcystin synthetase	[28]

HEPF	TTTGGGGTTAACTTTTTTGGGCATAGTC	57	52	472	Microcystin/nodularin synthetase	[23]
HEPR	AATTCTTGAGGCTGTAAATCGGGTTT	55	52	472	Microcystin/nodularin synthetase	[23]

PKS M4	GAAGCTCTGGAATCCGGTAA	52	55	650	Cylindrospermopsin polyketide synthase	[29]
PKS M5	AATCCTTACGGGATCCGGTGC	56	55	650	Cylindrospermopsin polyketide synthase	[29]

M13	GGCAAATTGTGATAGCCACGAGC	57	55	597	Cylindrospermopsin peptide synthetase	[29]
M14	GATGGAACATCGCTCACTGGTG	57	55	597	Cylindrospermopsin peptide synthetase	[29]