Table 2.
Binding of primer-templates to P. furiosus DNA polymerase
| Primer-template | KD (nM)a |
|---|---|
| Control (T) | 182 ± 23 (270) |
| U | 4.4 ± 1.3 (1.5) |
| diFBz | 52 ± 8 |
| FBzIm | 176 ± 19 |
a
The binding constants for the interaction of primer-templates (sequences given in the materials and methods) containing the bases indicated at the + 4 position were measured by direct titration (figure 5). The values (± standard deviation) are the averages found from four determinations. The values given in brackets for T and U were measured previously for analogous primer-templates using competitive titration (3).