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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nature. 2010 Jun 20;466(7302):68–76. doi: 10.1038/nature09204

Figure 4. Specificity within the ATG8 sub-network.

Figure 4

a, In vitro validation. S-tagged proteins were tested for GST-ATG8 binding (Supplementary Fig. S10). Green: binding. Red: no binding. b, LDS dependence. As in panel a using wild-type, GABARAPY49A/L50A or MAP1LC3BF52A/L53A proteins (Supplementary Fig. S11a,b). Blue edges: loss of binding. Green edges: binding maintained. Red edges: no binding with WT. c, ATG8ΔGly dependence. Wild-type and ATG8ΔGly proteins from 293T cells were subjected to LC-MS/MS. Blue edge: decreased binding. Red edge: increased binding. (Supplementary Fig. S12a, Table S3).