Figure 2.

Immunostaining for AKR1B1 and AKR1B10 in human eye tissues. A donor eye (77-year-old male) was treated with antibodies to AKR1B1 and AKR1B10 or preimmune control serum. Immune complexes were visualized by treatment with a horseradish peroxidase-conjugated secondary antibody and signal developed using diaminobenzidine tetrahydrochloride (DAB) to give a brown color. Tissues examined include cornea (a)–(c), lens (d)–(f), and retina (g)–(i). Immunostaining was particularly strong in the inner plexiform (IPL) and outer plexiform (OPL) layers. The inner nuclear layer is shown (INL).