Figure 2.

Primary Screen. HUVEC cells were transfected with 20 pmol of the combined Human siARRAY® SMARTpool® siRNA Libraries for Kinases (targeting 779 genes) and Cell Cycle Regulators (targeting 111 genes). Cell viability was analyzed 72 hrs post-Nucleofection®. (A) Representation of robust Z-scores of cell viability measures from 1 screening experiment. (B-E) Robust Z-scores of all primary hits with MAD of >3 in all three (B), two of three (C), or one of three (D) independent experiments and with MAD of <3 in all three experiments (E). (F) Robust Z-scores of the top 37 primary hits (with MAD of >3) from three independent experiments.