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. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Biochemistry. 2010 Jun 15;49(23):4766–4778. doi: 10.1021/bi100020e

Figure 3. PKCε affects Raf-1, ERK1/2 and STAT3 phosphorylation and prolongs and maximizes activation of the ERK1/2 pathway.

Figure 3

NIH-3T3 cells were transfected with the PKCε expression vector or empty CMV vector for 24 h, then treated with or without PMA for 24 h and examined by immunoblot for the effect on p-Raf-1 (A), pERK1/2 (B), and p-STAT3 Tyr705 (C). (D) Immunoblot analysis of pERK1/2 levels in NIH-3T3 cells treated with PMA and overexpressing the PKCε expression vector or empty CMV vector for the indicated times. (E) Densitometry of the data shown in (D), obtained from three independent experiments. In (E), immunoreactive bands were normalized to HPRT, and are expressed as an increase relative to control, (CMV samples were normalized to CMV at time zero (white bars), and PKCε samples were normalized to PKCε time zero (black bars). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control. Data are representative of at least three independent experiments.