Skip to main content
. Author manuscript; available in PMC: 2011 Jun 15.
Published in final edited form as: Biochemistry. 2010 Jun 15;49(23):4766–4778. doi: 10.1021/bi100020e

Figure 5. PKCε mediates TSPO protein levels and regulates c-Jun and STAT3 activity in MA-10 cells.

Figure 5

Effect of PKCε knockdown on TSPO expression, phosphorylation levels of c-Jun at Ser73 and STAT3 at Ser727 and Tyr705. Cells were seeded and treated with the siRNA pool specific for PKCε, or with scrambled siRNA as mentioned in materials and methods. Cells were analyzed for PKCε, c-Jun pSer73 (A), STATpSer727, STATpTyr705, and STAT3 by immunoblotting 96 h after siRNA transfection (C). Densitometric analysis of the blots was performed and immunoreactive bands were normalized to GAPDH (B) or Tubulin (D) controls, and data are expressed as decrease relative to control. Immunoblots are representative of at least two independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control.