Skip to main content
. Author manuscript; available in PMC: 2010 Jul 12.
Published in final edited form as: Virology. 2007 Apr 3;364(1):112–122. doi: 10.1016/j.virol.2007.03.006

Fig. 7.

Fig. 7

TGMV AL4 plays a role in AL2 and AL3 expression. The ability of promoter-reporter constructs containing mutations within the AL4 coding region to direct AL2 (A) and AL3 (B) expression was determined by comparing GUS activity to background (pTGA26; ~4% activity). Protoplasts (5 × 105 cells) were transfected with 10 μg of a promoter-reporter construct, and significant differences in GUS activity (ANOVA (P<0.05) measured in extracts isolated three days post-transfection. Columns represent relative GUS activity as compared to a wild type (AL1+/AL4+) DNA template, which was arbitrarily assigned a value of 100, from either three (A) or two (B) independent experiments.