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. Author manuscript; available in PMC: 2011 Jun 8.
Published in final edited form as: Curr Biol. 2010 May 13;20(11):1048–1052. doi: 10.1016/j.cub.2010.04.025

Figure 4. RanGTP Releases Somatic H1 from Nuclear Import Receptors and Promotes its Chromosome Binding.

Figure 4

(A) Top: Coomassie-stained gel of proteins pulled down with GST (G) or H1M- and H1A-GST fusion proteins (M, A) from metaphase-arrested extract. GST (22 kD) is not shown. Bottom: GST-H1A pull-downs supplemented with 15 μM RanQ69L (+) or buffer control (−).

(B) Representative mock- or H1M-depleted chromosomes assembled with or without recombinant H1A (1.2 μM) and RanQ69L (15 μM). A subpopulation of unrescued chromosomes was also observed in the +H1A +RanQ69L condition but is not shown.

(C) Average chromosome morphology (n > 30 per condition) comparing the effects of RanQ69L with either 1.2 μM H1A or 0.3 μM H1M. Doses were chosen that could not rescue chromosome morphology on their own. Error bars, standard error. See also Figure S3.