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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Mol Cancer Ther. 2010 Jun 1;9(6):1688–1696. doi: 10.1158/1535-7163.MCT-09-0919

Fig. 6. ARC/ABT-737-induced apoptosis in human cancer cells is dependent on caspase-3 and –9, but not on caspase-8 activity.

Fig. 6

A. Specific inhibitor for caspase-3, caspase-9 and pancaspase inhibitor reduce ARC/ABT-737 induced apoptosis, while specific inhibitor for caspase-8 demonstrates no reduction in apoptosis detected by immunoblotting for cleaved caspase-3 in SW480 and HepG2 cells. B. Quantitation of apoptotic cells by using in vitro chromatin condensation detection kit. Combination of ARC/ABT-737 enhance fluorescence intensity in SW480 cells, pan-caspase inhibitor, caspase-3 and -9 inhibitors, but not caspase-8 inhibitor lower the fluorescence intensity of SW480 cells (mean ± SD of 3 separate determinations).