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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Dec 27;17(1):112–116. doi: 10.1038/nsmb.1724

Figure 4.

Figure 4

STIM1 activates ORAI1 channels in membrane vesicles from S. cerevisiae. The experiment was carried out as in Figure 3b except for the use of D3cpV sensor. Fluorescence emission spectra were obtained from vesicles containing wild-type ORAI1, ORAI1 R91W or ORAI1 E106Q, either with no addition or following addition of STIM1CT (2.6 μM). The bar graph plots mean fluorescence intensity ratio F528nm/F475nm ± s.e.m. for each condition. STIM1CT changes the ratio significantly for wild-type ORAI1 (P < 0.001, two-tailed Welch’s t-test), but not for ORAI1 R91W and ORAI1 E106Q.