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. Author manuscript; available in PMC: 2010 Jul 12.
Published in final edited form as: Nat Genet. 2009 Jul 20;41(8):891–898. doi: 10.1038/ng.420

Figure 4. Accumulation of RS in ATRS/S MEF.

Figure 4

a, Growth curves of ATR+/+ and ATRS/S MEF. b, Percentages of cells at the G2 stage of the cell cycle derived from the analysis of DNA content by flow cytometry. c, Distribution of γH2AX and 53BP1 on ATR+/+ and ATRS/S MEF. Scale bar indicates 5 μm. d, Average number of chromosome breaks per cell on control and Seckel metaphases. f, Percentage of the Fra8E1 alleles that were found to be at a break by FISH analyses. e, Representative images of the type of genomic aberrations found on ATRS/S MEF. Metaphases were stained with probes recognizing the telomeres and the Fra8E1 fragile site. 50 metaphases were analyzed per condition in 3 replicates.