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. 2008 Feb 14;17(11):1540–1555. doi: 10.1093/hmg/ddn042

Figure 3.

Figure 3.

Figure 3.

Transmission electron microscopy of AxD white matter, R239H/- GFAP mutant mouse brain and GFAP aggregate-containing U251 cells. (A) Typical autophagic vacuoles (AVs) in RF-containing astrocytes in L359V AxD brain. A-I, abnormal astrocyte process containing RFs. A-II, a typical autophagosome, with double-limiting membranes, adjacent to the skein of GFAP intermediate filaments, as shown in boxed area in A-I. A-III, another instance of affected astrocytes with RFs and multiple vesicular vacuoles. A-IV, A-V: autophagosomes close to RFs. A-VI, A-VII, vacuoles with single-limiting membranes. (B) A representative autophagosome in a RF-containing astrocyte in a R236H/- GFAP mutant mouse brain (B-II), as shown in boxed area in B-I. Note vesicular structures are located in the vicinity of RFs and the skeins of GFAP filaments (B-I). (C) GFAP filaments and AVs in GFAP aggregate-containing U251 cells. Note that AVs were frequently seen in mt cells (C-IV), and occasionally in wt GFAP expressing cells (C-III). C-V, VI,VII,VIII, XI and X, representative AVs in mt GFAP expressing U251 cells. * filaments; →, autophagosomes; ▴, Rosenthal fibers or GFAP aggregates; ♂, vacuoles with single-limiting membranes, reflecting autolysosomes; #, multilamellar bodies. Scale bar: 0.5 µm, Magnification: 25 000×.