Figure 11.

Interactions of atlastin family members with the SPG4 protein spastin. (A and B) Yeast two-hybrid tests showing interactions of the spastin bait construct with full-length atlastins (A) and the indicated atlastin-1 deletion constructs, with residues 1-558 representing the full-length atlastin-1 protein (B), as revealed by growth selection on -His/Leu/Trp plates. The -Leu/Trp panel demonstrates similar transformation efficiencies for each reaction. Sequential 10-fold dilutions of yeast are shown for all panels. Strength of interaction was also assessed semi-quantitatively by determining the time for colonies to turn blue in X-gal filter lift assays: +++, <30 min; ++, 30–60 min, +, 60–120 min; and −, no significant activity.