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Atlastin-2 and -3 localize to ER membranes and along microtubules by immunogold electron microscopy. Electron microscopic analysis of HeLa cells immunostained for endogenous atlastin-2 (top and middle panels) or atlastin-3 (bottom panel) reveals gold particles not only at ER and VTCs/cis-Golgi (top and bottom panels), but also decorating membranes along microtubules (middle panel) (G, Golgi apparatus; MT, microtubules). Bars, 100 nm.
