Figure 8.

VSVG trafficking is not impaired in cells lacking both atlastin-2 and -3. HeLa cells were transfected with control siRNA (left panels), or with both atlastin-2 and -3 siRNAs (DKD (double knockdown); right panels), and both cell groups were then re-transfected with ts045 VSVG-GFP. The VSVG can be seen at perinuclear pre-Golgi and/or Golgi structures in both cell groups 60 min after moving to a permissive temperature. VSVG trafficked to the plasma membrane by 180 min in control cells as well as atlastin-2 and -3 DKD cells. Bars, 10 µm.